Molecular epidemiology of coxsackievirus A16 associated with hand, foot and mouth disease in Fujian province, China from 2020 to 2023
10.3760/cma.j.cn112866-20240611-00094
- VernacularTitle:福建省2020—2023年手足口病相关柯萨奇病毒A组16型的分子流行病学研究
- Author:
Linfeng LI
1
;
Shujing LI
;
Wenxiang HE
;
Yuwei WENG
;
Ying ZHU
;
Wei CHEN
Author Information
1. 福建医科大学公共卫生学院，福州 350100
- Keywords:
Hand, foot and mouth disease;
Coxsackievirus A16;
Molecular epidemiology;
B1c cluster
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(5):547-551
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the molecular epidemiological characteristics of coxsackievirus A16 (CVA16) in Fujian province from 2020 to 2023.Methods:The epidemiological characteristics of CVA16 associated hand, food and mouth disease (HFMD) in Fujian province from 2020 to 2023 was analyzed. The complete VP1 gene of CVA16 was amplified by RT-PCR and then sequenced, and genetic evolution was analyzed by MEGA X and other softwares.Results:From 2020 to 2023, there were 13 120 cases of HFMD in Fujian province, and the proportion of HFMD which caused by CVA16 was 16.5% (2 160/13 120). From 2020 to 2023, the proportion of accounted cases was 4.7% (94/2 019), 14.1% (457/3 243), 47.6% (1 521/3 199) and 1.9% (88/4 659) respectively. HFMD caused by CVA16 was mainly concentrated in children aged 1 to 5 years, and most of them were 3 years old. The genetic evolution and genotype analysis of 92 complete VP1 gene sequences obtained from 2020 to 2023 showed that the genetic distance between CVA16 strains in Fujian province and the prototype strain was far away. The CVA16 genotype in Fujian province from 2020 to 2023 has three clusters of B1a, B1b and B1c, among which the composition ratio of B1a and B1b in Fujian province in 2020 was 40% and 60% respectively. In 2021, B1a and B1b accounted for 81.8% and 18.2% respectively. Only B1a in 2022; in 2023, there were B1a, B1b and B1c, which respectively accounted for 44.4%, 7.4% and 48.2%. During the period from January to September, B1a was the main cluster. After October we observed an emergence of B1c cluster, which had never been found in Fujian province and was rare in China, was detected and became the dominant cluster.Conclusions:The evolutionary cluster of CVA16 dominant changed from B1b in 2020 to B1a in 2021-2023. After October 2023, the newly discovered B1c became the dominant cluster in Fujian province.
