Complete genome sequence and molecular characterization of a human respiratory syncytial virus subtype B strain isolated in Qinghai province
10.3760/cma/j.cn112866-20240605-00091
- VernacularTitle:青海1株人呼吸道合胞病毒B亚型的全基因序列测定及其分子特征分析
- Author:
Lifang HE
1
;
Mengqi YIN
;
Shengcang ZHAO
;
Zhijian TANG
;
Youju LEI
;
Jinying QI
;
Deng TIAN
;
Chunxiang WANG
Author Information
1. 青海省疾病预防控制中心，西宁 810007
- Keywords:
Human respiratory syncytial virus;
Subtype B;
Whole genome;
Molecular characteristics
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(5):533-538
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the genetic characteristics of the complete genome of a strain of human respiratory syncytial virus (HRSV) in Qinghai province in 2024.Methods:A total of 300 samples were collected during 2024 influenza surveillance in Qinghai province sentinel hospitals from patients with fever accompanied by severe respiratory infection symptoms. We used real-time fluorescent quantitative reverse transcription polymerase chain reaction RT-PCR) method to screen out HRSV subtype B (HRSVB) positive specimens, whole genome sequencing was performed on positivespecimens meeting the requirements for the sequencing. After downloading the global representative HRSVB genotypes at GenBank database, sequence alignment was performed, related evolutionary tree was built and the calculation and analyses of genetic distance were done, analyses of HRSVB sequencing of sequence homology of nucleotides, amino acids and amino acid mutation were performed.Results:The first strain in Qinghai, China/qinghai/2024-03 had a complete sequence of 15 140 bp nucleotides, with HRSV′s all structural characteristics, and subtype HRSVA prototype strain Long strains of nucleotide the lowest homology was 80.0%, and subtype HRSVB prototype strain nucleotide homology was above 94.7%. The result indicated that the first strain in Qinghai belonged to HRSVB subtype. Genetic evolution shows China/qinghai/2024-03 and USA/WA-S23450/2021 (OR326803.1) and Germany/2021 (OR795235.1) all belong to a branch, they have the closest relationship. Phylogenetic analysis of G gene showed that the strain belonged to BA9 genotype of HRSVB subtype, and the hypervariable regions of the genome were SH and G genes.Conclusions:In this study, the complete genome sequence of HRSV China/qinghai/2024-03 was obtained for the first time, and the basic molecular structural characteristics were elucidated, which filled the gaps in the gene and amino acid data of HRSV in our province, and also provided a basis for HRSV epidemiology.
