Molecular evolution of human infection with H9N2 subtype avian influenza virus in Anhui province from 2013 to 2022
10.3760/cma.j.cn112866-20240326-00042
- VernacularTitle:2013—2022年安徽省人感染H9N2亚型禽流感病毒分子进化分析
- Author:
Yidan XIA
1
;
Sitian YANG
;
Minhao HU
;
Xue ZHOU
;
Junling YU
;
Wanrong LUO
;
Weixi FANG
;
Jun HE
Author Information
1. 安徽医科大学公共卫生学院，合肥 230032
- Keywords:
H9N2;
Avian influenza virus;
Systematic evolution;
Molecular characteristics
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(5):527-532
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the genetic and molecular characteristics of H9N2 subtype avian influenza viruses from external environment and humans in Anhui Province from 2013 to 2022.Methods:Environmental samples and human samples were collected from Anhui influenza surveillance network laboratory. Sixty-three strains of virus were isolated in chicken embryos. RT-PCR was used to amplify the virus and whole genome sequencing was performed. To construct gene evolutionary tree and analyze its genetic characteristics and potential glycosylation sites.Results:The hemagglutinin (HA) gene belongs to the 9.2.4.5 clade, and the protein cleavage sites are mostly " PSRSSR\GL". The neuraminidase (NA) gene, basic protein-1(PB1) gene, acidic protein (PA) gene, non-structural protein (NS) gene and nucleoprotein (NP) gene belong to the F/98 clade, the matrix protein (MP) gene belongs to the G1/97 clade, and the basic protein-2 (PB2) gene belongs to the ST/7488 clade. Mutations of T155N, R164Q, H183N, T189D/V, A190V/T and Q226L occurred in HA protein, deletion of NA protein occurred at 62-64 sites, and mutations of T271A, I292V/M and E627V/L occurred in PB2 protein. At the same time, mutations of K356R and S409N occurred in the PA protein.Conclusions:The H9N2 subtype avian influenza viruses collected from external environment and human sources in Anhui province from 2013 to 2022 belong to the same evolutionary branch, and amino acid site mutations suggest that the virus shows a tendency to gradually adapt to the mammalian host environment. Therefore, further studies on the adaptive evolution of the virus and related monitoring work are needed.
