Genetic characterization of varicella-zoster virus in Jilin province from 2010 to 2023
10.3760/cma.j.cn112866-20240523-00087
- VernacularTitle:2010—2023年吉林省水痘-带状疱疹病毒流行株基因特征分析
- Author:
Xiang LI
1
;
Leilei WEI
;
Biao HUANG
;
Tao CHENG
;
Yuanchun SHAN
;
Guixiang QIN
;
Hongyan SUN
;
Shangwei JI
;
Xin TIAN
;
Simei FU
;
Shuang WANG
Author Information
1. 吉林省疾病预防控制中心（吉林省预防医学科学院），长春 130062
- Keywords:
Varicella-zoster virus;
Single nucleotide polymorphism;
Genotype;
Clade
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(5):521-526
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to analyze the genomic characteristics of Varicella-Zoster Virus (VZV) strains circulating in Jilin province from 2010 to 2023.Methods:Vesicle fluid from 78 sporadic cases with VZV infection were collected in Jilin province from 2010 to 2023, after detecting by Real-time PCR, 26 specimens (CT<25) were detected by PCR. Open reading frame 22(ORF22), ORF38 and ORF62 were amplified and analyzed. Genotyping was confirmed by SNPs ORF22 (37902, 38019, 38055, 38081 and 38177) and ORF38 (69424). Vaccine strains were indentified from wild-type strains according to ORF38 (69349) and ORF62 (106262, 107252, and 108111). Sequences were analyzed by homologous comparison and phylogenetic analysis.Results:The comparison with Dumas sequence revealed that SNPs (37902, 38055, 38081 and 38177) in ORF22 and ORF38 (69424) have mutations similar to the pOka strain, which belong to clade 2. Compared to the Dumas and Baike strains, all 26 samples were wild-type strains. JL2016-4 strain changes from threonine to asparaginyl at position 38059, JL2021-4 strain changes from arginine to proline at position 37933, from aspartic acid to tyrosine at position 37935, and from aspartic acid at base 38031 to tyrosine. JL2023-1 strain changes from arginine to leucine at position 37933.Conclusions:VZV has been prevalent for 14 years in Jilin province. The main epidemic strains belong to the clade 2. We should strengthen the monitoring of VZV outbreaks and raise the coverage rate of VZV vaccination.
