Correlation of polymorphism of the coding region of glutathione S-transferase M1 to susceptibility of nasopharyngeal carcinoma in south China population
10.3321/j.issn:1000-467X.2009.01.002
- VernacularTitle:谷胱甘肽硫转移酶M1基因编码区多态性与中国南方人群鼻咽癌易感性的关系
- Author:
He YING
1
;
Zhou GANG-QIAO
;
Li XIN
;
Dong XIAO-JIA
;
Chai XIAN-QI
;
Yao KAI-TAI
Author Information
1. 南方医科大学附属南方医院
- Keywords:
nasopharyngeal neoplasm;
genetic susceptibility;
glutathion Stransferase M1(GSTM1)gene;
glutathione S-transferase polymorphism
- From:Chinese Journal of Cancer
2009;28(1):6-10
- CountryChina
- Language:Chinese
-
Abstract:
Background and Objective：Glutathione S-transferase M1 (GSTM 1)deficiency may increase the risk of nasopharyngeal carcinoma (NPC).This study was to evaluate the correlation of the single nucleotide polymorphism (SNP) in the coding region of GSTMl gene to NPC susceptibility in southern China population.Methods：In total 239 NPC patients and 286 age-matched healthy controls were entered into the study.Among them.225 out of 239 NPC patients and 273 out of 286 controls were used for statisticaI analysis.SNP screening of all exons.relevant intron-exon boundaries.and the promoter region of GSTM 1，in total 4739bp，was performed by PCR direcl sequencing.The loci T1270533G and C1256088Cwere selected for the case-controI study using the tetra-Primer ARMS-PCR.as well as the sequencing method.Results：In totaf 29 SNPs of GSTM 1 were identified by sequencing.Missense mutation occurred in the polymorphic loci of T1270533G and C1256088C.However.no evident relationships between the variant of T1270533G and clinicaI phenotypes of NPC were obsewed in the NPC group and healthy control group(OR=0.1 70，95%CI=0.95-0.306for homozygote TT).The deletion frequency of C1256088C was 45%(45/100)for NPC patients and 42%(42/100)for controls.Conclusions：The polymorphism of T1270533G does not affect the detoxification function of GSTM1.The T1270533G JOCUS has no apparent association with genetic susceptibility to NPC in the southern China population.The IOSS rate of C1256088C is high in this study.
