Circadian Variation of Plasma Cortisol and Whole Blood Reduced Glutathione Levels in Nasopharyngeal Carcinoma Patients
10.3321/j.issn:1000-467X.2008.03.003
- VernacularTitle:鼻咽癌患者外周血血浆可的松水平和全血还原型谷胱甘肽含量的昼夜节律
- Author:
Ming-Wei WU
1
;
Zhao-Lei ZENG
;
Su LI
;
Ling GUO
;
Jin-Xin ZHANG
;
Fang QIU
;
Li-Jian XIAN
Author Information
1. 中山大学肿瘤防治中心
- Keywords:
KEYWORDS:Chrono-therapy;
Circadian rhythm;
Cortisol;
Glutathione;
Nasopharyngeal neoplasm
- From:Chinese Journal of Cancer
2008;27(3):237-242
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND & OBJECTIVE:Glutathione is involved in cellular protection against radiation damage and drug detoxification.This study was to investigate the circadian variation of plasma cortisol and whole blood reduced glutathione(GSH)levels in nasopharyngeal carcinoma (NPC)patients to provide references for chronotherapy for NPC.METHODS:A total of 13 NPC patients and 14 healthy volunteers were involved.Peripheral venous blood was sampled every 4 h during one 24hour period starting at 12:00 am.The plasma cortisol concentration was determined by radioimmunoassay;the GSH concentration was determined by high performance liquid chromatography.RESUL-TS:Plasma cortisol levels of both groups displayed clear and similar circadian rhIythms.The cortisot level peaked in both groups in the morning and was the lowest at mid-night.In both groups,GSH concentrations showed significant differences according to sampling time(ANOVA for Repeated Measures,F=5.18,P=0.02).By cosinor analysis.the circadian variation of the GSH Ievel in NPC group was marginally statistically significant(Cosinor analysis,P=0.06)with the acrophase appeared at 05:02:the GSH Ievel in control group displayed an obvious circadian rhythm and the acrophase appeared at 07:44+01:56(P＜0.01).The rhythm-adjust mean value of glutathione was(19.60+1.11)nmol/mg protein in NPC group and(8.95,±0.46)nmol/mg protein in control group.CONCLUSIONS:The circadian rhythm of the plasma cortisol level is maintained in NPC patients,even in some patients at advanced stages.In NPC patients,GSH shows a trend of circadian variation which is similar to that in healthy controls.These results could be used to select special schedules for chrono-radiotherapy and chrono-chemotherapy for NPC patients.
