Feasibility of monitoring clopidogrel resistance with flow cytometric analysis of platelet va-sodilator stimulated phosphoprotein phosphorylation
- VernacularTitle:流式细胞术检测VASP的磷酸化程度可监测氯吡格雷抵抗
- Author:
Guanghua LI
1
;
Yanfei LUO
;
Ying LUO
;
Ting LIN
;
Xiaobin FAN
;
Ling LIANG
;
Hongdong XIE
;
Jinwei HUANG
Author Information
1. 广东省人民医院//广东省医学科学院检验科
- Keywords:
flow cytometry;
coronary artery disease;
clopidogrel resistance;
platelets;
vasodilator-stimulated phosphoprotein
- From:
Journal of Southern Medical University
2014;(3):434-437
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the feasibility of monitoring clopidogrel resistance with flow cytometric analysis of platelet vasodilator stimulated phosphoprotein (VASP) phosphorylation. Methods Twenty patients with coronary heart disease (CHD) and 17 healthy volunteers were examined for platelet aggregation rate and phosphorylation of VASP (calculated as platelet reactivity index, PRI) using traditional optical nephelometry and flow cytometry before and after concurrent therapy of clopidogrel and aspirin. Results No significant differences were found in PRI between CHD group and healthy control group [(89.45 ± 5.22)%vs (86.58 ± 4.35)%] before treatment. The PRI in CHD group was significantly lowered after treatment to (67.66 ± 19.77)%(P<0.05). Clopidogrel resistance was found in 6 (30%) cases in CHD group by flow cytometric analysis, which showed a higher sensitivity than optical nephelometry (10%). Conclusion Flow cytometric analysis of VASP phosphorylation is a more reliable test to specifically evaluate clopidogrel resistance.
