Knockdown of survivin Expression by Small Interfering RNA Induces Apoptosis in Human Breast Carcinoma Cell Line MCF-7
10.3321/j.issn:1000-467X.2005.03.003
- VernacularTitle:利用siRNA阻抑survivin基因表达诱导乳腺癌细胞系MCF-7细胞凋亡的研究
- Author:
Ji-Xia LI
1
;
Ke-Yuan ZHOU
;
Tong LIANG
;
Yue-Fei ZHANG
Author Information
1. 广东医学院
- Keywords:
Gene therapy;
Cell apoptosis;
RNA interference;
Small interfering RNA;
Breast neoplasms
- From:Chinese Journal of Cancer
2005;24(3):268-272
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND & OBJECTIVE; survivin, a member of inhibitor of apoptosis protein (IAP) gene family, expresses in various human cancer tissues, and may facilitate tumor cell evasion from apoptosis, and promote aberrant mitotic progression. This study was to investigate cell proliferation and apoptosis status of human breast carcinoma cell line MCF-7 after knockdown of survivin. METHODS; Small interfering RNA was transfected into MCF-7 cells to inhibit expression of survivin. mRNA and protein levels of survivin were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Proliferation inhibition rate of MCF-7 cells was analyzed by MTT assay. Cell apoptosis was assayed by flow cytometry (FCM). RESULTS; The expression of survivin in siRNA-transfected group decreased by 64% in comparison to untransfected group. After treatment of different concentrations of siRNA, proliferation inhibition rate and apoptosis rate of MCF-7 cells were increased. The highest proliferation inhibition rate was 60.9%, and the highest apoptosis rate was 29.0% after treatment of 200 nmol/L of siRNA. CONCLUSION; survivin siRNA might be a useful therapeutic agent for the treatment of breast carcinoma.
