Research progress in digital PCR for the detection of occult hepatitis B virus infection
10.3760/cma.j.cn112866-20240309-00035
- VernacularTitle:数字PCR在隐匿性乙肝病毒感染检测中的研究进展
- Author:
Chen ZHANG
1
;
Xiangyan HUANG
Author Information
1. 山东第二医科大学医学检验学院，潍坊 261053
- Keywords:
Occult hepatitis B virus infection;
Digital PCR;
Fluorescence quantitative PCR;
Blood transfusion safety
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(3):351-356
- CountryChina
- Language:Chinese
-
Abstract:
Hepatitis B virus (HBV) infection is a major public health problem worldwide. Occult hepatitis B virus infection (OBI) is a special type of HBV infection with extremely low viral load, posing a potential threat to transfusion safety and great challenges for clinical diagnosis. Currently, detecting HBV DNA in the liver and/or blood is an effective method for diagnosis of OBI. In HBV DNA detection, compared with fluorescence quantitative PCR (qPCR), digital PCR (dPCR) provides a more efficient detection platform and is a reliable method for quantifying extremely low levels of nucleic acids. It has shown great application prospects in OBI detection. This article mainly reviews the common detection method for OBI, as well as the principles and advantages of dPCR, and outlines the current application status of dPCR in OBI detection. In the hope that dPCR can fully leverage its advantages in OBI detection and better serve the detection of blood donors and clinical diagnosis in the future.
