Construction of TCRβ repertoires in children with SARS-CoV-2 infection using high-throughput sequencing technology
10.3760/cma.j.cn112866-20240102-0003
- VernacularTitle:高通量测序技术构建新型冠状病毒感染儿童T细胞受体β链免疫组库
- Author:
Xiaoyu YI
1
;
Feng HE
;
Miao FENG
;
Zonghui XIAO
;
Hailan YAO
Author Information
1. 首都儿科研究所生化免疫研究室，北京 100020
- Keywords:
TCRβ repertoires;
High-Throughput Sequencing;
SARS-CoV-2;
Child Infection
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(3):326-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct T cell receptor β (TCRβ) repertoires in children with SARS-CoV-2 infection, and analyze the differences in TCRβ repertoires between children with SARS-CoV-2 infection and healthy children.Methods:Whole blood samples from 5 children infected with SARS-CoV-2 Delta variant and from 5 healthy children were collected. After RNA quality inspection and repertoires construction, high-throughput sequencing was conducted to analyze the differences in clonal expansion and diversity indices of the TCR repertoires between children infected with SARS-CoV-2 and healthy children. The frequency of use of TCRβ VJ genes was statistically analyzed using unpaired T-tests.Results:We successfully constructed the TCRβ repertoires of children infected with SARS-CoV-2 using high-throughput sequencing technology. The diversity index of the TCR repertoire in children infected with SARS-CoV-2 (9.78±1.23) was significantly lower compared to that in healthy children (13.40±2.12) ( P<0.05), and the TCR clonal expansion index in children infected with SARS-CoV-2 (0.18±0.07) was significantly higher compared to that in healthy children (0.06±0.06) ( P<0.05). A preliminary comparison of the frequency of use of TCRβ repertoire VJ genes found that, in children infected with the SARS-CoV-2, the most common V and J genes were TRBV28 and TRBJ2-1, respectively. Conclusions:The construction of the TCRβ repertoires in children infected with SARS-CoV-2 using high-throughput sequencing technology has revealed characteristic features of the TCRβ repertoires in these children. This is of significant reference value for unveiling the characteristics of the T-cell repertoires in children infected with SARS-CoV-2 and for the rapid construction of TCRβ immunological repertoires in other viral infections.
