Establishment of rapid influenza virus detection technology based on an integrated system
10.3760/cma.j.cn112866-20240328-00044
- VernacularTitle:基于一体化系统流感病毒快速检测技术的建立
- Author:
Xiaoshan ZHANG
1
;
Jing MA
;
Yanzhe HAO
;
Jing YANG
;
Meiling HOU
;
Hongxia LI
;
Jingduan LIU
;
Haodi MENG
;
Yuxi CAO
;
Xiaoguang ZHANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所，北京 102206
- Keywords:
Influenza virus;
Nucleic acid amplification testing;
Point of care test
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(3):319-325
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to develop a rapid and accurate integrated nucleic acid detection method tailored for the influenza virus.Methods:We designed primers and probes targeting the predominant influenza virus strains circulating in China in recent years. These were integrated with extraction and amplification reagents and a point of care testing (POCT) system to facilitate a seamless and expedited process involving nucleic acid extraction, reaction system preparation, amplification, and result interpretation for the influenza virus. The specificity of the POCT system was evaluated using cultured influenza viruses, while its cross-reactivity was assessed against common respiratory pathogens, including adenovirus and respiratory syncytial virus.Results:Our study successfully developed duplex amplification primers and probes for both influenza A and B viruses, achieving a detection threshold as low as 500 copies/ml. Specificity tests confirmed that the detection reagents did not show cross-reactivity with other respiratory pathogens such as adenovirus and respiratory syncytial virus. The POCT-based rapid nucleic acid detection method for influenza virus was established, it is capable of completing the entire process from nucleic acid extraction to amplification and result interpretation within 50 minutes, while enabling real-time data upload.Conclusions:The POCT-based rapid influenza virus detection kit developed in this study offers a " sample in, results out" convenience, making it suitable for rapid influenza virus detection in primary care settings. This innovation has significant potential for clinical application.
