Monitoring results of SARS-CoV-2 in urban sewage in Chongqing city in 2023
10.3760/cma.j.cn112866-20240329-00048
- VernacularTitle:2023年重庆市城市污水新冠病毒监测结果
- Author:
Gaomin LIU
1
;
Jiqin ZHENG
;
Yao CHEN
;
Wenge TANG
;
Zhifeng LI
;
Shujun NIE
Author Information
1. 重庆市疾病预防控制中心，重庆 400707
- Keywords:
Aluminum hydroxide adsorption-precipitation method;
Sewage monitoring;
SARS-CoV-2;
Multiple real-time fluorescent RT-PCR
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(3):294-298
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To offset the shortage of traditional large-scale population surveillance and provide early-warning signals in the early stage of the outbreak of COVID-19, people in Chongqing had carried out severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) monitoring since 2023.Methods:After COVID-19 was managed with measures against Class B infectious diseases, we selected five sewage treatment plants with automatic sample collection facilities in four districts of the main city. Two samples of sewage from each sewage treatment plant were collected every week. Then SARS-CoV-2 from these samples was concentrated by aluminum hydroxide adsorption-precipitation, detected and analyzed by multiple real-time fluorescent RT-PCR.Results:From January 16 to December 31 of 2023, a total of 496 sewage samples were monitored, of which 285 samples were positive by SARS-CoV-2 nucleic acid assay, with a total detection rate of 57.46%. The detection rate of SARS-CoV-2 in weeks 3-5, 18-21 and 40-47 was 100.00%. The daily mean nucleic acid concentration of SARS-CoV-2 in sewage peaked in the 18th week, and then began to decline, entering a low level and fluctuated in epidemic period. The variable trend of daily mean concentration of SARS-CoV-2 nucleic acid was basically consistent with daily number of SARS-CoV-2 infected patients or SARS-CoV-2 positive rate in fever clinic counted by infectious disease monitoring system.Conclusions:The detection rate of SARS-CoV-2 in sewage of Chongqing is relatively high, especially in April to May, and sewage monitoring can indirectly reflect the status of COVID-19 infection.
