Characterization of rhinovirus in patients with respiratory tract infection in Mianyang, 2021—2022
10.3760/cma.j.cn112866-20240227-00031
- VernacularTitle:2021—2022年绵阳地区流感样病例样本中的鼻病毒特征分析
- Author:
Yue GONG
1
;
Ming PAN
;
Guo CHEN
;
Qinqin SONG
;
Yanhai WANG
;
Chen GAO
;
Zhiqiang XIA
;
Lulu WANG
;
Qian CHENG
;
Kexin ZONG
;
Jun HAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 传染病溯源预警与智能决策全国重点实验室，北京 102206
- Keywords:
Influenza-like cases;
rhinovirus;
pathogenic characterization;
genetic evolution
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(3):279-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the prevalence and characteristics of Rhinovirus (HRV) infection in influenza-like Illness (ILIs) patients in Mianyang, Sichuan province, China.Methods:Throat swabs were collected from patients of ILIs in sentinel hospitals in Mianyang during 2021—2022. Real-time fluorescence quantitative PCR was used to detect 16 common pathogens. The VP4/VP2 coding region genes of HRV positive samples were amplified by nest PCR. The phylogeny, consistency and amino acid variation of different serotypes were analyzed and compared with reference sequences from GenBank database.Results:A total of 332 ILIs′ samples were collected with a virus detection rate of 58.73% (195/332) in Mianyang. Among them, 23 samples (23/332) were HRV-positive, and 18 VP4/VP2 sequences of HRV strains were successfully amplified. It was found that 13 HRV serotypes were detected in ILIs samples in Mianyang, which belonged to three genotypes, namely HRV-A (12 strains), HRV-B (5 strains) and HRV-C (1 strain).Conclusions:HRV was one of the pathogens of ILIs cases in Mianyang during 2021—2022, with HRV-A types as the dominant strains.
