Effect of microwave on adenovirus capsid integrity and antigenicity of hexon protein in simulated infectious wastes
10.3760/cma.j.cn112866-20240223-00026
- VernacularTitle:微波对模拟感染性废物中腺病毒衣壳完整性及Hexon蛋白抗原性的影响
- Author:
Kuo WANG
1
;
Shujun DING
;
Dandi LI
;
Xiaobin YANG
;
Zeming QIN
;
Li ZHAO
;
Liuying TANG
Author Information
1. 山东大学齐鲁医学院公共卫生学院微生物检验学系 山东省"十三五"高等学校感染性疾病防控重点实验室，济南 250012
- Keywords:
Microwave;
Infectious waste;
Adenovirus;
capsid;
Inactivation mechanism
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(3):247-251
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of microwave on human adenovirus type 2 (HAdV-2) capsid protein in simulated infectious wastes.Methods:Droplets of HAdV-2 virus suspension were added to medical disposable gloves to simulate infectious waste, irradiated under different microwave conditions, the temperature change was recorded, and the irradiated viral supernatant was treated with Dnase I and detected by PCR and qPCR to determine the effect of microwave on the integrity of the viral capsid. ELISA was used to detect the effect of microwave irradiation on the structure of viral hexon protein. The virus was treated alone at the highest temperature during microwave irradiation to investigate whether there were non-thermal effects during microwave disinfection.Results:The maximum temperature during microwave irradiation was 76 ℃, and the PCR and qPCR result showed that Dnase I could significantly damage the viral nucleic acid after microwave irradiation, while the virus control group and heat treatment group were not significantly affected, indicating that microwave irradiation could destroy the integrity of the viral capsid. The result of ELISA showed that microwave irradiation could significantly weaken the binding ability of Hexon protein and antibody, and the result of heat treatment group were similar.Conclusions:Microwave irradiation can destroy the integrity of the HAdV-2 capsid and the structure of Hexon protein, in which the damage to the integrity of the capsid is mainly due to non-thermal effects, and the structural changes of hexon protein are mainly due to thermal effects.
