Expressions of immune-associated molecules in serum of patients infected with respiratory syncytial virus subtype A
10.3760/cma.j.cn112866-20240321-00039
- VernacularTitle:A亚型呼吸道合胞病毒感染患者血清中免疫相关分子的表达研究
- Author:
Yi SHEN
1
;
Qiange MA
;
Tao LI
;
Guangcheng XIE
Author Information
1. 承德医学院病原生物学教研室，承德 067000
- Keywords:
Respiratory syncytial virus subtype A;
Cytokine;
Soluble toll-like receptor;
Soluble tumor necrosis factor receptor
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(3):239-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the changes of immune-associated molecules in serum of patients infected with respiratory syncytial virus subtype A (RSV-A).Methods:Serum specimens were collected from patients with respiratory infection at Department of Pediatrics of NO. 2 Clinical Teaching Hospital of Chengde Medical University during August 2022 to June 2023. The serum specimens were set as RSV-A single infection group and RSV-A coinfection group based on the detected pathogens in these patients. Serum levels of Th1 cytokines including interferon γ (IFN-γ) and tumor necrosis factor-α (TNF-α), and Th2 cytokines including interleukin 4 (IL-4) and IL-10, soluble toll-like receptor 2 (sTLR2) and 4 (sTLR4), soluble tumor necrosis factor receptor 1 (sTNFR1) and sTNFR2 were determined using enzyme-linked immunosorbent assay (ELISA) kits.Results:Serum levels of IFN-γ ( H=10.030, P=0.007) and IL-4 ( H=10.246, P=0.006) were significantly up-regulated in RSV-A single infection group and RSV-A coinfection group, however, levels of TNF-α ( F=1.154, P=0.322) and IL-10 ( F=1.738, P=0.188) were not significantly up-regulated. Area under the curve (AUC) of IFN-γ and IL-4 were 0.866 (95% CI: 0.767-0.966, P=0.002) and 0.986 (95% CI: 0.956-1.000, P=0.001), respectively. The levels of sTLR2 ( H=1.165, P=0.559) and sTLR4 ( H=1.657, P=0.437) were not significantly changed. Levels of sTNFR1 ( H=11.431, P=0.003) and sTNFR2 ( F=3.411, P=0.041) were significantly up-regulated in RSV-A coinfection group and the concentration of sTNFR2 was higher than sTNFR1. Concentration of sTNFR1 was peaked in RSV-A coinfected with bacterium group and RSV-A coinfected with dual bacteria group. AUC of sTNFR2 was 0.967 (95% CI: 0.911-1.000, P=0.002). Conclusions:Expressions of immune-associated molecules, including IFN-γ, IL-4, sTNFR1 and sTNFR2 were significantly changed in patients infected with RSV-A, and these immune-associated molecules maybe engage in the interaction and pathogenesis between RSV-A and host.
