Sequence analysis of whole genome of influenza A(H3N2) virus in Huai′an
10.3760/cma.j.cn112866-20231221-00076
- VernacularTitle:淮安市H3N2亚型流感病毒全基因组序列的遗传特征分析
- Author:
Pengfei YANG
1
;
Fang HE
;
Wanli YIN
;
Tong GAO
;
Wang ZHANG
;
Mingyue WEI
;
Chenglong XIONG
;
Wei HU
Author Information
1. 淮安市疾病预防控制中心，淮安 223002
- Keywords:
Influenza A;
H3N2;
Whole genome analysis;
Genetic evolution
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(2):156-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the biological traits and mutations of the influenza A (H3N2) virus in order to produce a vaccine and offer references for controlling and preventing influenza epidemics.Methods:Four strains of the influenza A(H3N2) virus were chosen from the Huai′an surveillance network laboratory. Nucleic acid extraction, library building, and sequencing (CridION x5 MKI Nanopore) were used to produce the whole-genome sequences. Using homologous alignments of whole-genome sequences, phylogenetic tree construction, and amino acid variant screening, bioinformatics analysis was carried out.Results:The nucleotide identity between 8 gene segments ranged from 97.1% to 100.0%. The gene that differed the most from the reference sequences was HA (97.1%-99.9%), and the gene that differed the least was MP (98.6%-99.9%). The HA gene (3.06%) and MP gene (1.43%) were the regions with the greatest and lowest frequencies of nucleotide site change, respectively. The rates of nucleotide change varied significantly between the genes ( χ2=14.293, P=0.046). Four influenza A(H3N2) virus strains′ whole-genome phylogenies from each of the eight gene segments maintained a roughly consistent topological structure. One strain was linked to the 3C.2a1b.1b clade, which was lost at the 142NWT, 149NGT(HA1), and 436NLS(NA). Three strains were linked to the 3C.2a1b.2a.1a clade lineage. Amantadine and NA inhibitors were effective against all Huai′an strains. Conclusions:The antigenicity of one strain of Huai'an strain changed and its matching with the vaccine strain of that year was low. It is suggested that the genetic surveillance of H3N2 influenza virus should be continuously strengthened to provide scientific basis for influenza prevention and control and influenza vaccine screening.
