Epidemiological characteristics of HFMD and gene characteristics of Coxsackievirus A10 VP1 region in Linyi City in 2023
10.3760/cma.j.cn112866-20240119-00010
- VernacularTitle:2023年临沂市手足口病流行特征及柯萨奇病毒A10型VP1区基因特征分析
- Author:
Chunyun JIANG
1
;
Shengxiang JI
;
Chuanbao WANG
;
Xiangliang LIU
;
Deqing YIN
;
Ge ZHANG
Author Information
1. 临沂市疾病预防控制中心，临沂 276001
- Keywords:
Hand, foot and mouth disease;
Coxsackievirus A10;
VP1 region;
Genetic characteristics
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(2):150-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the pathogenic situation of hand, foot and mouth disease (HFMD) in Linyi City, and to analyze the gene characteristics of Coxsackievirus A10 complete VP1 region.Methods:The samples of HFMD cases from Linyi City in 2023 were tested for typing and strain isolation, and the VP1 gene of CV-A10 isolate was amplified and sequenced. The obtained sequences were compared with those in the NCBI database, and the phylogenetic tree was constructed for gene characteristics and molecular epidemiological analysis.Results:In 2023, a total of 861 samples of HFMD were collected, and 594 (68.99%) were positive for nucleic acid tests. The male to female ratio of positive cases was 1.56∶1. Children under 5 years old accounted for 81.65%, and the highest incidence season was from June to August (83.84%). CV-A6 was the main pathogen (84.51%), followed by CV-A10 (9.93%). The nucleotide and amino acid homology of VP1 gene sequence among 13 isolates were 93.29%-100.00% and 97.65%-100.00%, respectively. The nucleotide and amino acid homology with AF081300-Kowalik/USA/1950 was lower (75.95%-76.62%, 91.72%-92.41%). The amino acid and nucleotide homology with C2c was the highest (94.28%-96.76%, 98.28%-100.00%), and the genetic distance was the closest (0.04-0.06). Amino acid site variation analysis showed that compared with the prototype strain AF081300-Kowalik/USA/1950, the isolates had more site variation, while only some isolates had I80V, E141K, P147S, T219I, E240K and V261I mutations compared with the representative strain C2c. The genetic evolution tree showed that the isolates were all in the same clade as the C2c representative strains, and they all belonged to the C2c genotype, and the isolates were further divided into two smaller clades.Conclusions:In 2023, CV-A6 was the main pathogen of HFMD in Linyi City, followed by CV-A10. All CV-A10 isolates were C2c genotypes and can be divided into two evolutionary clades. Continuous monitoring and genetic characterization of CV-A10 should be strengthened.
