Analysis of the whole genome sequence of a GⅡ.12P16 norovirus strain
10.3760/cma.j.cn112866-20231109-00054
- VernacularTitle:一株GⅡ.12[P16]型诺如病毒毒株的全基因组序列分析
- Author:
Meijia LI
1
;
Guoqiang WANG
;
Mingxin GUO
;
Xiaolin LIU
;
Ti LIU
;
Wenkui SUN
;
Zhongyan FU
;
Zengqiang KOU
Author Information
1. 山东第一医科大学（山东省医学科学院）公共卫生与健康管理学院，济南 250117
- Keywords:
Norovirus;
Genome;
Recombination
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(2):144-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To characterize the complete genome sequence and elucidate the structural features of norovirus (NoV) isolate SD20200267.Methods:The viral nucleic acid was extracted from patient samples, followed by amplification and sequencing for genotyping based on the nucleotide sequences. The metagenomic sequencing technology was utilized for whole genome sequencing, and subsequent analysis was performed on the acquired nucleotide sequences.Results:The complete genome sequence of the SD20200267 strain, spanning a total length of 7 465 nucleotides, was successfully obtained. The SD20200267 strain belongs to the GⅡ.12 and GⅡ.P16 genotypes in the VP1 and RdRp regions, respectively. The nucleotide sequence identity of SD20200267 strain with other GⅡ.12[P16] strains ranged from 96.0% to 97.3%, exhibiting 15 amino acid variations. The strain displayed evidence of recombination, with the recombination site located in the overlapping region of ORF1 and ORF2.Conclusions:SD20200267 is classified as a GⅡ.12[P16] strain, and recombination was observed in the overlapping region of ORF1 and ORF2.
