Effect of siRNA targeting HDAC1 gene on proliferation, apoptosis, histone acetylation, and histone metylation in gastric cancer cells in vitro
- VernacularTitle:RNA干扰沉默HDAC1基因对胃癌细胞增殖、凋亡、组蛋白乙酰化和甲基化的影响
- Author:
Hanxu ZHUANG
1
;
Xudong MA
;
Yadong LAI
;
Xiangnong XU
;
Xiaozhong WANG
Author Information
1. 福建医科大学附属漳州市医院消化内科
- Keywords:
gastric cancer;
histone acetylation;
HDAC1;
RNA interference
- From:
Journal of Southern Medical University
2014;(2):246-250
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of silencing histone deacetylases1 (HDAC1) gene by RNA interference on the proliferation, apoptosis and histone modulation in gastric cancer MGC-803 cell line. Methods The optimal segment targeting HDAC1 gene was designed and transfected into MGC-803 cells by Lipofectamine TM2000. HDAC1 mRNA and protein in the transfected cells were detected by RT-PCR and Western blotting, respectively. The growth inhibition of MGC803 cells was evaluated by MTT assay and the cell apoptosis was detected with TUNEL assay. The expression of Bcl-2, procaspase-9, procaspase-3, c-Myc, histone acetylation of H3, H4, and histone methylation of H3K9 was detected by Western blotting. Results The siRNA targeting HDAC1HDAC1 markedly suppressed mRNA expression, inhibited cell proliferation and induced apoptosis of MGC-803 cells in a concentration manner. Transfection of the cells with HDAC1 siRNA at 0, 30, 60, and 120 nmol/L for 24 h resulted in a cell apoptotic rate of (4.8±2.7)%, (18.5±3.5)%, (41.4±4.3)%, and (59.2±5.5)%, respectively, and caused down-regulation of the expressions of Bcl-2, proCaspase9, proCaspase3 and c-Myc, upregulation of histone acetylation of H3, H4, and down-regulation of histone methylation of H3K9. Conclusion Silencing HDAC1 gene expression with HDAC1 siRNA can promote histone H3 and H4 acetylation and inhibit histone methylation of H3K9 to suppress the proliferation and induce apoptosis of gastric cancer MGC-803 cells.
