Analysis on the detection and genotypes distribution of norovirus in environmental sewage in Fujian province during 2022-2023
10.3760/cma.j.cn112866-20240127-00016
- VernacularTitle:2022—2023年福建省监测点环境污水中诺如病毒的检测和型别分布特征分析
- Author:
Shiqi YAN
1
;
Mengping ZHANG
;
Hairong ZHANG
;
Bingshan WU
;
Dong LI
;
Zhifei CHEN
;
Xiuhui YANG
Author Information
1. 福建医科大学公共卫生学院，福州 350001
- Keywords:
Environmental surveillance;
Norovirus;
Sewage;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(2):131-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the detection status and genotypes distribution characteristics of norovirus(NoV)in environmental sewage from three monitoring points in Fujian province, and to explore the significance of its application to NoV monitoring.Methods:Sewage samples were collected monthly at 5 sampling sites in representative monitoring cities, enriched and concentrated. Partial gene fragments of norovirus VP1 were amplified by reverse transcription-semi nested polymerase chain reaction (RT-snPCR), TA cloned and sequenced. Genotypes were identified based on the sequencing.Results:A total of 56 sewage samples were collected from July 2022 to June 2023. The detection rates of GⅠ and GⅡ were 89.29% (50/56) and 94.64% (53/56), respectively. A total of 7 NoV GⅠ genotypes and 13 GⅡgenotypes were identified. GⅠ.1, GⅠ.4, GⅡ.4 and GⅡ.17 were the dominant genotypes. NoV genotypes detected in different sampling sites were not exactly the same. The detection rate of NoV was low from August to November 2022, and the prevalence of the dominant genotypes was different in different seasons. GⅠ.1 and GⅡ.4 were highly prevalent from August to November 2022, but were replaced by GⅠ.4 and GⅡ.17 from December 2022 to June 2023, respectively. More NoV genotypes were detected in January-June 2023, comparing to the July-December 2022. The dominant genotype GII.17, has multiple clades and new variants have been discovered that are different from the 2014/2015 circulating strains.Conclusions:The detection rates of NoV in environmental sewage were very high, and genotypes were diverse. Environmental sewage surveillance could be an important complementary method for NoV cases surveillance.
