In vitro anti-respiratory syncytial virus activity of interferon-α2b and interferon-λ1
10.3760/cma.j.cn112309-20230116-00012-1
- VernacularTitle:干扰素α2b和λ1体外抗呼吸道合胞病毒活性研究
- Author:
Enrui GUAN
1
;
Qian ZHANG
;
Aijun CHEN
;
Chao WANG
;
Yiman HUANG
;
Fenlian MA
;
Lishu ZHENG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 传染病溯源预警和智能决策全国重点实验室 国家卫生健康委员会医学病毒与病毒病重点实验室，北京 100052
- Keywords:
Respiratory syncytial virus;
IFN-α2b;
IFN-λ1;
Human airway epithelium
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(2):117-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze antiviral activity against respiratory syncytial virus (RSV) of interferon (IFN)-α2b and IFN-λ1 on Hep2 cells and human airway epithelial (HAE) cells.Methods:IFN-α2b or IFN-λ1 was incubated with Hep2 cells after RSV infection, and 48 hours later, the cytopathic effect was observed, the viral load was determined using real time/reverse transcription quantitative polymerase chain reaction (RT qPCR), RSV F protein expression was detected using immunofluorescence, and cell survival rate was detected using crystal violet. HAE cells were incubated with IFN-α2b or IFN-λ1 for 24 hours, and then HAE were challenged with RSV. The viral load in the culture supernatant was determined on days 1-7 using RT qPCR, RSV F protein was determined with immunofluorescence and the viral titers in the culture supernatant was detected on day 7 by plaque assay.Results:In Hep2 cells, the CPE of the treatment groups (IFN-α2b and IFN-λ1) was alleviated compared to the virus control group, and the CPE of the high concentration group was lighter than that of the low concentration group. Different concentrations of IFN-α2b and IFN-λ1 could significantly reduce the viral load of RSV ( P<0.001), and the viral load of the high concentration group was significantly lower than that of the low concentration group ( P<0.001). In addition, IFN-α2b and IFN-λ1 could reduce the RSV F protein expression after RSV infection and improve cell survival rate. In HAE cells, IFN-α2b and IFN-λ1 could inhibit RSV virus replication, reduce virus titers ( P<0.001) and reduce RSV F protein expression. Conclusions:IFN-α2b and IFN-λ1 both showed great antiviral activity against RSV in Hep2 and HAE cells, providing data reference for the study of interferon against respiratory viruses.
