Feasibility of continuous extracorporeal normothermic liver perfusion using autologous blood:a study in pigs
- VernacularTitle:自体猪血体外常温持续灌流离体肝脏的可行性分析
- Author:
Kaimin XIANG
1
;
Huaxu YU
;
Jinzhong YUAN
;
Zheng LI
Author Information
1. 中南大学湘雅三医院普外科
- Keywords:
porcine liver;
autologous blood;
extracorporeal perfusion;
hepatic failure;
organ preservation
- From:
Journal of Southern Medical University
2014;(2):223-227
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the feasibility of sustaining the viable status of a liver graft in at least 96 h by extracorporeal perfusion using autologous blood. Methods Eight extracorporeal porcine liver perfusions using autologous blood were performed, each for 96 h with hepatectomy, cold preservation, cannulation of vessels, and initiation of perfusion with normothermic oxygenated porcine blood. The graft viability was assessed by metabolic, synthetic, hemodynamic, and histologic parameters. Results After 96 h of normothermic, extracorporeal perfusion using autologous blood, the isolated livers maintained normal physiological levels of pH and electrolytes with sustained hepatic protein synthesis (complement and factor V) throughout the perfusion. Hemodynamic parameters maintained normal physiological ranges. Histological inspection demonstrated good preservation of the liver with a good architectural integrity. Conclusion It is possible to sustain the viable status of a liver graft within 96 h by extracorporeal perfusion using autologous blood.
