Point Mutation Analysis on Tumor Suppressor Genes in Stool of Patients with Colorectal Carcinoma by Silver Stain Polymerase Chain Reaction Single Strand Conformation Polymorphism
10.3321/j.issn:1000-467X.2001.06.010
- VernacularTitle:银染PCR-SSCP检测粪便中结肠直肠癌抑癌基因突变的研究
- Author:
Jin-Rong LIN
1
;
Bo JIANG
;
Hong LIN
;
Ya-Li ZHANG
;
Dian-Yuan ZHOU
Author Information
1. Guangzhou General Hospital of PLA
- From:Chinese Journal of Cancer
2001;20(6):608-611
- CountryChina
- Language:Chinese
-
Abstract:
Objective：The aim of this study was to seek a new approach for early detection and screening colorectal carcinoma. Methods: Point mutation analysis through 8％ non denaturant polyacrylamide gel electrophoresis(acrylamide∶ N,N, methylene bisacrylamide=29∶ 1) was applied to examine stool of patients with colorectal carcinoma. Results: Forty five patients with different histological categories at all clinical stages and normal cases were examined. There were 14 individuals with lymph node metastasis while thirty one without lymph node involvement. Of 45 samples investigated, twenty eight patients with adenomatous polyposis coli(APC) or/and mutated in colorectal cancer(MCC) mutations in the stools were identified, which representing 62.2％ of all patients and 93.3％ of the patients in whom the tumor tissue with gene mutations. APC or MCC determined in the stool was 91.3％ or 84.6％ respectively corresponding to the carcinomas in which APC or MCC aberrants had been detectable. There were no significant relationships between genetic alterations in the stools and histological types, Dukes stages,lymph node involvements, sizes or locations of the tumor (P >0.05), and between genetic abnormalities in the stools and histomorphologies or blood occult tests of the patients (P >0.05). Conclusions: The method presented here may provides a useful noninvasive technique for screening,detection and prognosis of colorectal neoplasms.
