miR- 221 mediates epithelial- mesenchymal transition- related gene expressions via regulation of PTEN/Akt signaling in drug-resistant glioma cells
- VernacularTitle:miR-221调控PTEN/Akt信号介导人胶质瘤耐药细胞上皮间质转化相关基因的表达
- Author:
Qiang XIE
1
;
Zuoping HUANG
;
Yongrong YAN
;
Feng LI
;
Xueyun ZHONG
Author Information
1. 暨南大学医学院病理系
- Keywords:
glioma;
miR-221;
PETN;
Akt;
epithelial-mesenchymal transition;
multi-drug resistance
- From:
Journal of Southern Medical University
2014;(2):218-222
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between miR-221 and epithelial-mesenchymal transition (EMT) in drug-resistant glioma cells. Methods The expression levels of miR-221, PTEN, p-Akt, E-cadherin, vimentin, and MRP1 were quantitatively analyzed in Z1 cells (primary drug-resistant cells), Z2 cells (drug-sensitive cells) and Z2-BCNU cells (drug-resistant cells) using fluorescent real-time PCR and Western blotting. Results The expression levels of PTEN were significantly increased in Z2 cells compared with Z1 and Z2-BCNU cells which overexpressed miR-221 and vimentin. The expression levels of vimentin, p-Akt and MRP1 were significantly decreased in Z2 cells overexpressing E-cadherin. Conclusion MiR-221 regulates the expression of EMT-related genes through down-regulation of PTEN and activation of PI3-K/Akt signaling.
