Correlation between Abnormal Expressions of CD44 Protein and Gene and Lymph Node Metastasis for Human Lung Carcinoma
10.3321/j.issn:1000-467X.2001.06.007
- VernacularTitle:人肺癌CD44蛋白和基因的异常表达与淋巴结转移的相关性
- Author:
Bin Lian ZHANG
1
;
E Yu SUN
Author Information
1. General Hospital of PLA
- From:Chinese Journal of Cancer
2001;20(6):595-598
- CountryChina
- Language:Chinese
-
Abstract:
Objective：The aim of this study was to investigate the abnormal expressions of CD44 protein and gene, relationship between the abnormal expressions of CD44 protein and gene and lymph node metastasis of human lung carcinomas. Methods： SP immunohistochemistry and RT PCR/Southern methods were used to investigate the expressions of CD44 protein and gene of human lung carcinomas. The immunohistochemistry results were quantitatively analyzed with QTM970 computed image analyzer. Results： SP immunohistochemistry results and quantitative analysis revealed that stronger expression of CD44 protein was observed in non small cell lung carcinomas than in para carcinomatous pulmonary tissues, in non small cell lung carcinomas with lymph node metastasis than in non small cell lung carcinomas without lymph node metastasis （ F=16.67, P< 0.01） . RT PCR/Southern results shew that more abnormal CD44 mRNA transcripts were observed in the non small cell lung carcinomas (NSCLCs) with lymph nodes metastasis than in non small cell lung carcinomas without lymph node metastasis（ χ 2=12.13,P< 0.01） . Conclusions： The abnormal expressions of CD44 protein and gene were observed in non small cell lung carcinomas. The abnormal expressions are not only involved in the development, but also in the lymph nodes metastasis of non small cell lung carcinomas. CD44 expressions may have significant clinical importance in the evaluation of the patient prognosis and the potential tendency of lymph nodes metastasis.
