Ca2 +-activated K + channel switching in smooth muscle participates in atherosclerosis development in diabetic rats
- VernacularTitle:平滑肌钙激活钾通道表达转换参与糖尿病大鼠动脉粥样硬化发生
- Author:
Yan WANG
1
;
Limei ZHAO
;
Xingli SU
;
Wei YU
;
Xiuling DENG
Author Information
1. 西安交通大学医学院生理学与病理生理学系
- Keywords:
diabetes;
vascular smooth muscle;
Ca2+-activated K+channel;
vascular complication
- From:
Journal of Southern Medical University
2014;(2):188-192
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in aorta morphology and Ca2+-activated K+ (KCa) channel expression in the diabetic rats. Methods A diabetic rat model was established by a single intraperitoneal injection of streptozotocin (30 mg/kg) after a modified high fat and glucose diet for 8 weeks. Pathological changes in the aorta were observed with HE staining, elastic fiber staining, Masson's trichrome staining and immunohistochemistry. Both the mRNA and protein levels of KCa channels in the aorta were measured by RT-PCR and Western blotting. Results Early atherosclerotic changes were observed in the aorta wall of the diabetic rats. The mRNA and protein levels of KCa1.1 channel α- and β-subunits were significantly decreased, while the expression of KCa3.1 channels was obviously enhanced in the middle layer of the aorta in the diabetic rats. Conclusion KCa channel switching in smooth muscles may play a role in the development of atherosclerosis in diabetic rats.
