Genome-wide molecular characterization of a rare group A rotavirus equine-like DS-1-like G3P 8 in China
10.3760/cma.j.cn112866-20231017-00043
- VernacularTitle:中国罕见A组轮状病毒DS-1样G3P[8]的全基因组分子特征分析
- Author:
Guangping XIONG
1
;
Yuhang WEI
;
Rui PENG
;
Jiaxin FAN
;
Xiaoping TANG
;
Zhimiao HUANG
;
Mengjie DONG
;
Ruyi CHE
;
Dandi LI
Author Information
1. 甘肃中医药大学公共卫生学院，兰州 730000
- Keywords:
Group A rotavirus;
Genome-wide;
DS-1-like G3P [8];
Molecular characterization
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(1):29-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective:A genome-wide molecular characterization of FJ21351116, a strain of G3P[8]-E2 2021 collected in Fujian, China, was performed.Methods:Whole genome sequencing of FJ21351116 was performed using a high-sensitivity group A rotavirus whole genome sequencing method. Genomic characteriza-tion of the virus was assessed by nucleic acid sequence analysis using MEGA 11.0, Geneious 9.0.2 and DNASTAR software. Neutralization epitopes of VP7 and VP4 (VP8*) were analyzed using BioEdit v. 7.0.9.0 and PyMOL v. 2.5.2.Results:In this study, FJ21351116 was shown to be a G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 genotype, and the result of phylogenetic tree showed that the VP7, VP4, VP3, and NSP2-NSP5 genes of the FJ21351116 strain were related to the equine-like DS-1-like G3P[8] genes that have been detected in Japan in recent years. VP6, VP1, VP2, and NSP1 genes are closely related to G2P[4] in most countries, especially in Singapore, suggesting that this strain was formed by genetic reassortment during the evolution of equine-like G3P[8] and G2P[4]. Evolutionary relationships between the VP7/VP4 genes of FJ21351116 and Rotarix and RotaTeq vaccines suggest that the multiple mutations in both VP7 and VP4 (VP8*) neutralizing antigenic epitopes and vaccine amino acid sites. It is hypothesized that the Rotarix and RotaTeq vaccines may be less effective against equine DS-1-like G3P[8] RVA, and the sequence differences with Rotarix are higher than those with RotaTeq.Conclusions:In this study, we found a rare case of DS-1-like G3P [8] RVA strain in China. Currently, horse-like DS-1-like G3P [8] RVA is relatively rare in China and may be poorly protected by vaccine strains, emphasizing the importance of continuous monitoring of RVA strains and the development of efficient and full-coverage RVA vaccines.
