Growth Suppression of Nasopharyngeal Carcinoma Cell through Transfection of BRD7 Gene
10.3321/j.issn:1000-467X.2001.06.002
- VernacularTitle:BRD7基因转染对鼻咽癌细胞生长的抑制作用
- Author:
Ying YU
1
;
Shi ZHU
;
Bi ZHANG
;
Hua Zhong LI
;
Juan-Juan XIANG
;
Ming ZHOU
;
Ling Xiao LI
;
Yuan Gui LI
Author Information
1. Central South University
- From:Chinese Journal of Cancer
2001;20(6):569-574
- CountryChina
- Language:Chinese
-
Abstract:
Objective：This study was designed to explore the effect of BRD7 gene negative associated with nasopharyngeal carcinoma (NPC) on the growth of NPC cell line HNE1. Methods： The mammal expression vector of BRD7, pcDNA3.1(＋ )/BRD7, was constructed and transfected into HNE1 cell. Stable G418 resistant clones were isolated, and the integration of the exogenous vector DNA and the expression of BRD7 gene were detected by Southern blot and RT PCR respectively. Finally the cytobiological characterization of positive clone (B 4) was analyzed by using population double time (PDT), soft agar assay, cytometry, and xenograft. Results： The PDT of G418 resistant HNE1 cell with epression of BRD7 was 53 h and significantly longer than that of vector transfected HNE1 cell and untransfected HNE1 (P< 0.01). Flow cytometric data shown that more BRD7 transfected cells went into phase G0－ G1 than controls. And it also presented decreased clonogenicity and tomorigenicity in soft agar assay and tumor formation in nude mice. Conclusion： The reexpression of BRD7 could favor the malignant phenotype revision of NPC cells. And BRD7 gene might be a good candidate of tumor suppressor gene correlated with NPC.
