Study on the relationship between HBV gene mutation and disease progression in patients with hepatitis B virus infection
10.3760/cma.j.cn112866-20231205-00068
- VernacularTitle:乙肝病毒感染者HBV基因突变与疾病进展的关系研究
- Author:
Suya HAN
1
;
Shuang ZHANG
;
Lin TANG
;
Qudong SU
;
Fuzhen WANG
;
Feng WANG
;
Hui ZHENG
;
Feng QIU
;
Hongyi LI
;
Yu WANG
;
Liping SHEN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委医学病毒和病毒病重点实验室，北京 102206
- Keywords:
Hepatitis B virus;
Genetic mutations;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(1):21-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the whole genome sequence and key site mutations of hepatitis B virus (HBV) in patients with different stages of disease progression, and to understand the relationship between HBV genetic characteristics and disease progression.Methods:Serum samples and basic information of hepatitis B patients with asymptomatic HBV carrier, chronic hepatitis B patients, cirrhosis patients and primary hepatocellular carcinoma patients were collected. Nested PCR was used to amplify the samples to obtain HBV whole gene sequences. Phylogenetic trees were constructed to determine the genotype of the samples, and gene mutations of the samples were analyzed combined with reference sequences of each type.Results:A total of 256 samples were successfully amplified, including 68 asymptomatic HBV carrier patients, 118 CHB patients, 15 LC patients and 55 HCC patients, and five genotypes (B, C, D, I and C/D) were detected. The result of comparative analysis showed that the mutation rate of 56 nucleotide sites was significantly different among the four groups ( P<0.05). In addition to the discovery of C105T, A1762T/G1764A and G1899A and other previously reported key site mutations, the mutation rates of T53A, C1485T and C1628T in newly diagnosed HCC group were significantly higher than those in other groups, and the mutation rates of T2150G and T2151C in asymptomatic HBV infection group were significantly higher than those in other groups. A total of 26 sequences were deleted, mainly distributed in the pre-C and pre-S regions. The deletion mutation rate in the HCC group was significantly higher than that in the other groups. Conclusions:The data of this study indicate that some nucleotide substitution mutations and deletion mutations may be closely related to the occurrence and development of HBV-related diseases, and HCC patients are more likely to have gene mutations than non-HCC patients. These result provide a reference for understanding the relationship between viral mutation and the progression of HBV infection-related diseases.
