Comparison of growth characteristics of different genotypes of Japanese encephalitis virus in different cell lines
10.3760/cma.j.cn112866-20231225-00073
- VernacularTitle:不同基因型别乙型脑炎病毒在不同细胞系上的生长特点比较
- Author:
Weijia ZHANG
1
;
Shenghui LIU
;
Xiaohui YAO
;
Yuke ZHENG
;
Jiehui WU
;
Ruichen WANG
;
Shihong FU
;
Qikai YIN
;
Fan LI
;
Kai NIE
;
Qianqian CUI
;
Songtao XU
;
Huanyu WANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 传染病溯源预警和智能决策全国重点实验室，北京 102206
- Keywords:
Japanese encephalitis virus;
genotypes;
cell lines;
growth characteristics
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(1):15-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the growth characteristics of different genotypes of Japanese encephalitis virus (JEV) in different cell lines, and to provide scientific basis for the selection of cell lines in the study of JEV.Methods:BHK-21, Vero, C6/36, PK-15, DF-1, N2a, SH-sy5y and MDCK cell lines were selected. The proliferation ability of genotype 1 (NX1889 strain), genotype 3 (P3 strain) and genotype 5 (XZ0934 strain) JEV in these cell lines was evaluated by plaque assay and RT-qPCR.Results:Significant cytopathogenic effects (CPE) were observed in BHK-21, Vero, C6/36, DF-1, N2a and PK-15 cell lines across all three JEV genotypes. However, no significant differences in CPE characteristics were observed within the same cell line. SH-sy5y and MDCK cell lines did not show significant CPE, but virus proliferation was detected in SH-sy5y cell line, while MDCK cell line were found to be insensitive to JEV. No significant difference was observed in the proliferation curves of G1, G3 and G5 JEV in BHK-21, Vero and SH-sy5y cell lines. In C6/36 and PK-15 cell lines, the titer of G1 JEV was higher than that of G3 and G5. In DF-1 cell line, G5 demonstrated a higher titer than the other two genotypes, whereas in N2a cell line, G5 showed a lower titer than the other two.Conclusions:There are differences in the proliferation of three different genotypes of JEV in different cell lines, which can provide reference for the study of JEV in different directions.
