Determination of Soluble Fas, Carcinoembryonic Antigen， and Interleukin-6 in Serum of Patients with Colorectal Carcinoma and Clinical Significance
10.3969/j.issn.1000-467X.2001.05.019
- VernacularTitle:大肠癌患者血清癌胚抗原、可溶性 Fas、白细胞 介素 -6水平测定及其临床意义
- Author:
Jia-Yin XIE
1
;
Gui-Yin SUN
;
Zhi-Bin LUO
;
Lian-Yang ZHANG
;
En-Ren LENG
Author Information
1. Daping Hospital the Third Military Medical University
- From:Chinese Journal of Cancer
2001;20(5):527-530
- CountryChina
- Language:Chinese
-
Abstract:
Objective： This study was designed to determine the serum carcinoembryonic antigen (CEA), soluble Fas (sFas), and interleukin-6 (IL-6) levels in colorectal cancer and analyze their clinical significance. Methods： Serum sFas and IL-6 were tested in pre-operated and post-operated 162 patients with colorectal cancer using double antibody sandwich ELISA method, serum CEA and immune indicators were also examined. Results： Levels of serum CEA, sFas and IL-6 [(32.52± 10.81)μ g/L, (20.97± 8.19) ng/L, (36.87± 11.20) ng/L, respectively] in the colorectal cancer patients with the positive rate (sFas： 53.7％ ;IL-6： 67.91％ ; CEA： 41.98％ ) were significantly higher than those in healthy control group and benign patients （ P<0.01), the post operated patients showed a lower levels CEA, sFas, and IL 6 than the pre operation patients （ P< 0.01） , serum levels of sFas and IL 6 in post operated patients were the lowest. Serum sFas level was correlated with tumor metastases, serum concentration of IL 6 was correlated strongly with tumor size and metastases (P< 0.01). Serum concentration of sFas was correlated negatively with lymphocyte transformated rate, NK or LAK killing rate, and had marked correlation among CEA, IL 6, and sFas. Conclusion： The positive rates of serum sFas and IL 6 in the colorectal carcinoma patients were higher than those of the serum CEA. Serum sFas and IL 6 levels reflected indirectly situation of proliferation and metastases in tumors. sFas may inhibit the patients immune function and tumor cells apoptosis, and IL 6 may accelerate proliferation of the colorectal carcinoma cells. These results indicate that combinative measuring of the serum levels of CEA, sFas, and IL 6 may be an useful method for clinical diagnosis, disease prognosis, and treatment effect in colorectal patients.
