Effects of metformin on human oral cancer KB cell proliferation and apoptosis in vitro
- VernacularTitle:二甲双胍对人口腔癌KB细胞增殖及凋亡的影响
- Author:
Fang WANG
1
;
Jincheng XU
;
Fei XIA
;
Zhe LIU
;
Surong ZHAO
;
Hao LIU
;
Zhiwen JIANG
Author Information
1. 蚌埠医学院第一附属医院
- Keywords:
oral cancer;
metformin;
apoptosis;
glucose-regulated protein 78;
caspase-3
- From:
Journal of Southern Medical University
2014;(2):159-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of metformin on the proliferation and apoptosis of human oral cancer cell line KB in vitro. Methods Human oral cancer cell line KB was exposed to different doses of metformin (0, 1.25, 2.5, 5, 10, and 20 mmol/L), and the changes in cell viability were detected using MTT assay. Colony formation of the cells was observed following an 8-day metformin exposure. The changes in mitochondrial membrane potential were measured by JC-1 assay, and PI staining was used to observe the cell apoptosis. Western blotting was employed to detect the changes in the protein expressions of GRP78 and activated caspase-3. Results Metformin exposure caused time-and dose-dependent suppression of KB cell proliferation, and exposure to 5 mmol/L metformin for 24, 48 and 72 h resulted in cell survival rates of 68.0%, 36.9%, and 14.5%, respectively. Metformin significantly inhibited KB cell colony formation. Exposure of the cells to increased concentrations of metformin gradually increased the apoptotic rate and decreased mitochondrial membrane potential. Metformin caused an initial up-regulation followed by a down-regulation of GRP78 expression in KB cells and increased the expression of activated caspase-3. Conclusion Metformin can inhibit the proliferation and induce apoptosis of KB cells, the mechanism of which may involve the activation of the mitochondrial apoptotic pathway and endoplasmic reticulum stress.
