Study on the Role of p53 Gene Transfer on Human Glandular Lung Cancer Cell Growth in Nude Mice
10.3321/j.issn:1000-467X.2001.05.011
- VernacularTitle:转染 p53基因对肺腺癌细胞株裸鼠 移植瘤生长的影响
- Author:
Ping LI
1
;
Bei-Ning WANG
;
Zhen-Ruo DING
Author Information
1. the General Hospital of Beijing Military Region
- From:Chinese Journal of Cancer
2001;20(5):496-498
- CountryChina
- Language:Chinese
-
Abstract:
Objective： This study was designed to explore the significance and the role of wild-type p53 (wt-p53) gene and mutant p53 gene(mt-p53) transfer on human glandular lung cancer cell growth in nude mice. Methods： wt-p53 gene and mt-p53 gene were transfected and lipofectin-mediated into the human glandular lung cancer cell line GLC-82. And the growth of gene-transfected cell lines were observed in vitro and in vivo. Results： The colony number in the colong-forming experiment and the volume and weight in nude mice were greater in the mf-p53 tranfecting cells group than in the control group. The tumor resulting from the cells transfected with the wt-p53 gene grew more slowly and was smaller than that from control GLC-82 cells. In contrast, the tumor from the cells transfected with the mt-p53 gene grew faster than that produced by cells transfeted with the wt-p53 gene and that produced by control GLC-82 cells. Conclusion： The wild-type p53 gene could inhibit the glandular lung cancer cell growth in nude mice and the mutant p53 gene could enhance the glandular lung cancer cell growth in nude mice.
