Comparison of immune effects of varicella zoster virus gE protein combined with different adjuvants in mice
10.3760/cma.j.cn112866-20230928-00037
- VernacularTitle:水痘带状疱疹病毒gE蛋白联合不同佐剂对小鼠免疫效果的比较
- Author:
Jiehui WU
1
;
Ruichen WANG
;
Shihong FU
;
Kai NIE
;
Fan LI
;
Qikai YIN
;
Ying HE
;
Guodong LIANG
;
Huanyu WANG
;
Hai LI
;
Songtao XU
Author Information
1. 中国医科大学，沈阳 110000
- Keywords:
Varicella zoster virus;
Glycoprotein E;
Adjuvants;
Immune effect
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(6):592-599
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study contrasts the immune efficacy of the varicella zoster virus glycoprotein E (VZV gE)using Al/CpG combined adjuvants and AS01 adjuvant in BALB/c mice.Methods:BALB/c mice were immunized at 0 and 21 days respectively, and serum antibodies were detected using enzyme-linked immunosorbent assay. Detection of neutralizing antibodies in mouse serum using varicella zoster virus; enzyme-linked immunosorbent spot assay was used to detect cellular immune response.Results:Following two intramuscular immunizations, mice in the experimental groups (Shingrix, gE+ Al/CpG, and gE+ AS01) demonstrated elevated neutralizing antibody titers and an augmented count of lymphocytes releasing IFN-γ and IL-4. The gE+ Al/CpG group displayed the highest neutralizing antibody titer (1943), yet the AS01-adjuvanted groups (Shingrix and gE+ AS01) showed increased lymphocyte counts secreting IFN-γ and IL-4 compared to the Al/CpG group (gE+ Al/CpG). In comparison to the AS01 adjuvant, Al/CpG adjuvants triggered a humoral immune response favoring Th2 in mice. The proportions of CD4 + T and CD8 + T cells were not significantly different among the experimental groups. Conclusions:Al/CpG adjuvant combined with gE protein resulted in high neutralizing antibody titers, while the intensity of the induced cellular immune response was inferior to that of AS01 adjuvant.
