Effects of HMG-CoA Reductase Inhibitors on Proliferation and Growth of Gastric Cancer Cells in Vitro
10.3321/j.issn:1000-467X.2001.05.005
- VernacularTitle:HMG-CoA还原酶抑制剂对胃癌细胞增殖的影响
- Author:
Xiao-Yun DING
1
;
Ding-Guo LI
;
Han-Ming LU
;
Qin-Fang XU
Author Information
1. the First Hospital of Ningbo
- From:Chinese Journal of Cancer
2001;20(5):474-476
- CountryChina
- Language:Chinese
-
Abstract:
Objective： This study was designed to investigate the effects of HMG-CoA reductase inhibitors on proliferation and growth of gastric cancer cells. Methods： MTT colorimetric assay and flow cytometry were used to measure the proliferative activity and the cell cycle progression of gastric cancer MNK450 cells. Results： Gastric cancer cells treated with Simvastatin had reductions in proliferative activity, S-phase fraction and proliferative index in a dose dependent manner with statistical significance at the concentrations of more than 1 μ mol/L as compared with normal control. The inhibitory rate ranged from 8.0％ to 51.5 ％ when cells were treated with Simvastatin at the concentrations of 0.2 -100 μ mol/L for 72 hours. Inhibition induced by Simvastatin was reversed by simultaneous addition of mevalonic acid at the concentation of 10 mmol/L. Gastric cancer cells were arrested by Simvastafin in G1 at the concentations of 1-20 μ mol/L. The fraction of S-phase cells was reduced from 32.1％ to 15.0％ after exposure to 20 μ mol/L Simvastatin for 36 hours. The addition of 10 mmol/L mevalonic acid overcome G1 arrest induced by 5 μ mol/L Simvastatin. Otherwise, the percentage of cells in G2 plus M2 remained rather constant throughout the treatment. Meanwhile, Pravastatin had no significant effect on the proliferatiive activity of gastric cancer MNK450 cells. Conclusion： Simvastatin could inhibit the proliferation and growth of gastric cancer cells, which suggests that Simvastatin has the effect of antitumor.
