Sequence Analysis of EBV-BARF0 Gene in Nasopharyngeal Carcinoma from Guangdong Area
10.3321/j.issn:1000-467X.2001.05.003
- VernacularTitle:鼻咽癌组织中 EBV-BARF0基因序列的测定
- Author:
Li-Bing SONG
1
;
Yi-Xin ZENG
;
Ying-Hong MA
;
Qing-Lun LIU
;
Man-Zhi LI
;
Duan LI
;
Hui-Min WANG
Author Information
1. Sun Yat-sen University of Medical Sciences
- From:Chinese Journal of Cancer
2001;20(5):464-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective： There were variations of EBV genome from different areas and they may affect the biologic function of EBV (such as LMP1). EBV-BARF0 was highly detected in the patients with nasopharyngeal carcinoma(NPC) and it s variations have not been reported. This study was designed to determine the sequence and variation of EBV-BARF0 gene in the patieats with NPC from Guangdong area. Methods： PCR was used to amplify the EBV-BARF0 gene in 20 patients with NPC and the productions were sequenced on the ABI377. Results： Comparing with standard B95-8, there were four loci with variances including： 160473 （ G→ T） , 160545 （ C→ T） , 160701 （ C→ A） , 160707 （ G→ C） of sequences and 2 （ Ala→ Ser） , 26 （ Leu→ Phe） , 78 （ Arg→ Ser） , 80 （ Ala→ Pro） of amino acid in 20 patients with NPC. Conclusion： Since the BARF0 gene of EBV is expressed in all nasopharyngeal carcinoma cell line and biopsies, but it is not expressed or less frequenly in lymphoma tissues and cell lines,the authors firstly reported the variation of EBV-BARF0 which compared with B95-8 in the patieats with NPC from Guangdong area. These suggest that the gene may play an important role in the carcinogenesis and development of nasopharyngeal carcinoma.
