Analysis of etiological molecular characteristics of an outbreak of acute hemorrhagic conjunctivitis
10.3760/cma.j.cn112866-20230702-00070
- VernacularTitle:一起急性出血性结膜炎暴发疫情病原学分子特征分析
- Author:
Junxian XU
1
;
Huanying ZHENG
;
Ting OUYANG
;
Biao ZENG
;
Wei ZHANG
;
Xiaoxian LU
;
Hanri ZENG
;
Wuyang SHI
;
Bixia KE
;
Meng ZHANG
;
Bosheng LI
;
Xiaoling DENG
Author Information
1. 南方医科大学公共卫生学院，广州 510515
- Keywords:
Acute hemorrhagic conjunctivitis;
CVA24v;
Phylogenetic analysis;
Mutations
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(5):497-504
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the evolutionary characteristics and variation of etiological agent in an acute hemorrhagic conjunctivitis (AHC) outbreak in a city of Guangdong province in May, so as to provide scientific basis for formulating a new round of measures for prevention and control of AHC epidemic.Methods:In this study, 20 conjunctival swabs were collected from AHC patients, and enterovirus, human enterovirus 70 (HEV70) and coxsackievirus A 24 variant (CVA24v) nucleic acids were detected by real-time fluorescence quantitative PCR. In addition, the VP1 and 3Cpro regions of the CVA24v positive samples were sequenced to analyze their evolutionary relationship with the CVA24v strains circulating in China and abroad.Results:All the 20 eye swab samples were EV-positive, and CVA24v-positive, with a positive rate of 100.00%, and all were HEV70-negative.The genomes of CVA24v in VP1 and 3Cpro regions of CVA24v in 5 and 7 samples were successfully sequenced. Based on molecular characterization analysis of VP1 and 3Cpro regions, it was found that the CVA24v isolated in this outbreak had the greatest nucleotide similarity with the CVA24v strains isolated in Thailand in 2014 and French Reunion Islands in 2015. The phylogenetic analysis of 3Cpro and VP1 regions showed that the CVA24v isolated in this outbreak is clustered together with the CVA24v that was prevalent in Thailand in 2014 and the French Reunion Islands in 2015, and have high affinity. Compared with CVA24v isolated in Guangdong in 2010, Thailand in 2014, and French Reunion Islands in 2015, CVA24v isolated in this outbreak was replaced at 4 amino acid sites in 3Cpro region and 1 amino acid site in VP1 region.Conclusions:The cause of this outbreak is enterovirus CVA24v, which has the highest similarity to CVA24v isolated in Thailand in 2014 and in the French Reunion Islands in 2015. There were new amino acid mutations in both 3Cpro and VP1 regions.
