Effects of Matrine on hTERT-mRNA Expression and Telomerase Activity in K562 Cells
10.3321/j.issn:1000-467X.2001.04.011
- VernacularTitle:苦参碱对K562细胞端粒酶hTERT-mRNA表达及其酶活性影响作用的研究
- Author:
Xu-Fen LI
1
;
Su-Zhan ZHANG
;
Shu ZHENG
;
Xing ZHANG
Author Information
1. 浙江大学附属第二医院
- From:Chinese Journal of Cancer
2001;20(4):391-393
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study was designed to investigate the effects of matrine on hTERT-mRNA expression and telomerase activity in K562 cells and their relationship. Methods: The hTERT-mRNA expression was determined by RT-PCR assay in untreated and matrine-treated K562 cells, and telomerase activity was analyzed by TRAP-PCR-ELISA assay. Results: The hTERT-mRNA expression of K562 cells was significantly inhibited when treatment with matrine, while telomerase activity was decreased. Conclusion: Matrine can inhibit the hTERT-mRNA expression and telomerase activity of K562 cells. Telomerase activity might be related to the expression of hTERT-mRNA.
