Significance of Multidrug Resistance-Associated Protein Gene and P-gp Expression in Malignant Lymphomas
10.3321/j.issn:1000-467X.2001.04.008
- VernacularTitle:多药耐药相关蛋白基因和P-糖蛋白在恶性淋巴瘤中的表达与意义
- Author:
Xi-Gui YANG
1
;
Ling WEI
;
Shu-Ping SONG
;
Li-Ya JIA
Author Information
1. Shandong Tumor Hospital Institute
- From:Chinese Journal of Cancer
2001;20(4):379-382
- CountryChina
- Language:Chinese
-
Abstract:
Objective： This study was designed to investigate the relationship between expression level of multidrug resistance-associated protein (MRP) or P-glycoprotein (P-gp) genes and chemotherapy efficacy or clinical drug resistance in the patients with malignant lymphomas. Methods： Using the methods of semi quantitative reverse transcription polymerase chain reaction (RT-PCR) and flow cytometry (FCM), The authors examined the expression level of MRP and P-gp of 46 lymphoma patients. Results： The expression level and positive rate of P-gp in the recurrent patients was higher than that in untreated patients (P<0.001). There was no difference in MRP gene expression level and positive rate between recurrent and untreated patients (P>0.05). Chemotherapeutic effective rate in P-gp positive patients (26.67％ ) was lower than that in P-gp negative patients (83.87％ )(P<0.001). While there was no difference between MRP gene positive and negative patients, their chemotherapy effective rate had no difference. Relevant analysis showed that there was no correlation between MRP and P-gp (r-0.0808, P>0.05). Conclusion： P-gp expression correlates to multidrug resistance, and is the major mechanism of clinical drug resistance of lymphomas, whereas, MRP gene does not appears to play any role in that course.
