Study of Anticancer Effect of Hydroxycampothecin-based Combination against Experimental Nasopharyngeal Carcinoma
10.3321/j.issn:1000-467X.2001.04.005
- VernacularTitle:以羟基喜树碱为基础的联合方案对实验性鼻咽癌抗瘤作用的研究
- Author:
Li-Wu FU
1
;
Yong-Ju LIANG
;
Qi-Chao PAN
Author Information
1. Sun Yat-sen University of Medical Sciences
- From:Chinese Journal of Cancer
2001;20(4):363-367
- CountryChina
- Language:Chinese
-
Abstract:
Objective: This study was designed to evaluate topoisomerase I inhibitor 10-hydroxycampothecin-based combination chemotherapy for nasopharyngeal carcinoma (NPC) in vitro and in vivo. Methods: Antitumor activity of 10-hydroxycampothecin (HCPT) in combination with 5-fluorouracil (5-FU) and/or cisplatin (DDP) in NPC cell line CNE2 and in CNE2 xenograft of nude mice were determined. The cytotoxicity was measured by MTT assay. Results: HCPT, 5-FU,and DDP had potential cytotoxicity to CNE2 cells. Their IC50 were 14.2 μ mol/L, 17.2 μ mol/L, 49.2 μ mol/L,respectively. HCPT in combination with DDP had significant anticancer effect for NPC in vitro and in vivo. But no significant additivity or synergism was showed in the combination of HCPT with 5-FU in vitro and in vivo. HCPT＋ DDP＋ 5FU was much better than HCPT＋ DDP for chemotherapy of NPC and the toxicity was endurable in the nude mice bearing CNE2 xenograft. Conclusion: HCPT＋ DDP and HCPT＋ DDP＋ 5-FU were potential regimen for chemotherapy of NPC.
