Tea Polyphenols Down Regulated Expression of Cyclin D1 in Nasopharyngeal Carcinoma Cells
10.3321/j.issn:1000-467X.2001.04.004
- VernacularTitle:茶多酚诱导鼻咽癌细胞cyclin D1表达下调
- Author:
Fei-Jun LUO
1
;
Zhi HU
;
Xiao-Rong ZHAO
;
Xi-Yun DENG
;
Wei YI
;
Huan-Hua GU
;
Ya CAO
Author Information
1. Hunan Medical University
- From:Chinese Journal of Cancer
2001;20(4):358-362
- CountryChina
- Language:Chinese
-
Abstract:
Objective： This stduy was designed to investigate the molecular mechanism of cell proliferation inhibited by tea polyphenols in nasopharyngeal carcinoma cell line CNE-LMP1. Method： Light microscope, MTT assay, flow cytometry, Western blot,and reporter gene analysis were applied to investigate the effects of tea polyphenols and(-)-epigallocatechin-3-gallate (EGCG) on the cell cycle and related molecular mechanisms. Result： After treatment of CNE-LMP1 cells with tea polyphenols(200 μ g/ml), the number of proliferating cells was obviously decreased as determined by light microscopy, and the survival rate of cells decreased from 100％ to 38.1％ as determined by MTT assay. With the increase of tea polyphenols concentrations, the number of cells in S-phase was obviously decreased from 22.20％ to 13.16％ (200 μ g/ml), and the number of cells in Phase G1 were elevated from 68.50％ to 74.08％ . It suggested that tea polyphenols could arrest the cell cycle at G1/S checkpoint. The transcription and the expression of cyclin D1 were obviously declined 4-5 fold. Furthermore, after treatment with tea polyphenols, transactivities of NFκ B and AP-1 was obviously down-regulated in CNE-LMP1 cells for 5-6 and 7-8 fold, respectively. Conclusion： Tea polyphenols could arrest cell cycle and inhibit cells proliferation of nasopharyngeal carcinoma, and down-regulation of the gene transcription and expression of cyclin D1 might also be involved in these events.
