Analysis of the mechanism of hepatitis B virus in one unpaid blood donor
10.3760/cma.j.cn112866-20221130-00246
- VernacularTitle:一例无偿献血者间歇性检出乙肝病毒DNA的血清学及S区基因特征分析
- Author:
Chao LIU
1
;
Kewang SUN
;
Hao WANG
;
Xiangyan HUANG
Author Information
1. 解放军联勤保障部队第九六〇医院输血医学科（解放军济南血液中心），济南 250031
- Keywords:
Blood donors;
Intermittent detection of HBV DNA;
Occult hepatitis B virus infection;
S gene;
Gene sequencing
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(4):414-417
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The serological characteristics and S gene sequencing of intermittent detection of hepatitis B virus (HBV) DNA from an unpaid blood donor were analyzed to provide a basis for correct interpretation of intermittent detection of HBV DNA from blood donors and better guarantee of blood safety.Methods:The samples of blood donors with intermittent detection of HBV DNA were collected for two blood donations. ELISA was used to detect HBsAg and NAT screening for HBV DNA. Electrochemical luminescence method was used to detect five items of hepatitis B. Nested PCR was used to amplify the HBV S gene, and gene sequencing was performed. Genotype and mutation were compared and analyzed.Results:HBsAg was negative in both samples by ELISA.NAT result were non-reactive once and reactive once. Hepatitis B e antibody (Anti-HBe) and hepatitis B core antibody (Anti-HBc) were positive. The same S gene sequence was obtained from two samples of the donor by amplification and sequencing, and the genotype was HBV genotype C. No mutation was found in the reference sequence comparison with S gene.Conclusions:The donor has occult HBV infection (OBI), which is manifested as intermittent detection by routine screening nucleic acid detection reagent, which is easy to miss HBV detection and seriously affects the safety of transfusion. Sensitive screening reagents and method are very important for the safety of blood transfusion.
