Research on the present situation of detection strategies for infectious markers related to transfusion transimission in China
10.3760/cma.j.cn112866-20230109-00003
- VernacularTitle:我国血站输血传播相关传染病标记物检测策略现状研究
- Author:
Wei TAN
1
;
Shengyan YING
;
Ning CHENG
;
Yujun LI
;
Xiaoli CHEN
;
Fang WANG
;
Yang ZHANG
;
Xiaojie LIU
;
Lin BAO
;
Yong DUAN
;
Chen MA
;
Chunlan LIU
;
Dengfeng WANG
;
Zhijun ZHEN
;
Li LI
;
Jian ZHANG
;
Ranran LU
;
Peng WANG
;
Mingxia LI
;
Xinli JIN
;
Xiaobo CAI
;
Mei YU
;
Jianling ZHONG
;
Lili ZHU
;
Jianping LI
Author Information
1. 南京红十字血液中心，南京 210003
- Keywords:
Blood station laboratory;
Nucleic acid test;
Mixed sample;
Individual-donation nucleic acid testing
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(4):383-388
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the detection strategy and basic detection situation of markers of infectious diseases transmitted by transfusion in blood testing laboratories of blood stations in China.Methods:Based on the data of practice comparison working party of Blood Stations in Mainland of China from 2017 to 2021, the data on the testing strategies and the basic detection information of the markers for the transmission of infectious diseases through transfusion in the member laboratories of the practice comparison working party of Blood Stations in Mainland of China from 2017 to 2021 were collected, and the situation of the selection for testing markers, testing strategy and the testing method and other relevant aspects were sorted out and analyzed by charts.Results:The selection of the testing markers was consistent, but HTLV testing item was added in some member laboratories. The detection strategy of using two ELISA reagents and one nucleic acid testing (NAT) reagent simultaneously was adopted in 47 member blood stations; 3) NAT method was dominated by mini pool-NAT in member laboratories. The number of members adopting mini-pools of 8 (MP8)-NAT decreased from 17 in 2017 to 14 in 2021, while the number of members adopting mini-pools of 6 (MP6)-NAT increased from 13 in 2017 to 22 in 2021; Roche NAT system accounted for the largest proportion.Conclusions:In order to ensure blood safety and avoid missing detection, the blood stations still adopt the detection strategy of using two ELISA reagents and one nucleic acid testing (NAT) reagent simultaneously; Meanwhile, in order to increase the NAT positive rate, the proportion of mini pool-NAT mainly decreased year by year despite its dominating role, while the proportion of individual donation-NAT increased year by year; NAT method is transiting from mini-pools of 8 (MP8) to mini-pools of 6 (MP6); The proportion of imported NAT system used in NAT laboratory is relatively large.
