Telomerase Activity and p53 Abnormality in Non-Small Cell Lung Cancer and Their Clinic Significance
10.3321/j.issn:1000-467X.2001.03.018
- VernacularTitle:端粒酶活性表达及p53异常在非小细胞肺癌中表达及临床意义
- Author:
Wei-Dong WEI
1
;
Zhi-Fan HUANG
;
Tie-Hua RONG
;
Yi-Long WU
;
Ming-Tian YANG
;
Qi-Wan LIANG
Author Information
1. Sun Yat-sen University of Medical Sciences
- From:Chinese Journal of Cancer
2001;20(3):301-304
- CountryChina
- Language:Chinese
-
Abstract:
Objective：This study was designed to investigate telomerase activity and p53 abnormality in the patients with nonsmall cell lung cancer (NSCLC) and their association. Methods：Telomeric repeat amplification protocol (TRAP) assay was used to detect the telomerase activity in NSCLC tissues, tumor-adjacent non-cancer lung tissues, lung benign lesions; Western blot was used to examine p53 protein in NSCLC. Results：Telomerase activity expressions frequency were：93.02％ (80/86) for NSCLC; 9.33％ (7/75) for tumor-adjacent non-cancer lung tissues;27.27％ (3/11) for lung benign lesions. Frequency of telomerase activity expression in NSCLC was much higher than that in tumor-adjacent non-cancer lung tissues or lung benign lesions, but was not significant different between histological types, differentiations, tumor size, and pathologic stages. The frequency of p53 abnormality in NSCLC was 51.42％ （ 36／ 70） . The differences of p53 abnormality between pathologic Stage Ⅰ -Ⅱ and Ⅲ -Ⅳ , Grade Ⅰ and Ⅱ -Ⅲ have statistic significance. But there was no significant association between the telomerase activity expression and p53 protein abnormality. Conclusions：In the markers of NSCLC, telomerase activity expression is more sensitive than p53 abnormality, but p53 is more helpful in pathologic stages and grades. In genesis and proceeding of NSCLC, telomerase activating and p53 abnormality maybe two independent factors.
