Up-Regulation Effect of 8-Br-cAMP on Expression of Connexin Gene Cx43 in Tongue Carcinoma Cell Line
10.3321/j.issn:1000-467X.2001.03.012
- VernacularTitle:8-Br-cAMP上调舌癌细胞株间隙连接蛋白基因Cx43表达的研究
- Author:
An-Xun WANG
1
;
Hong-Zhang HUANG
;
Qing-Yu KONG
Author Information
1. Sun Yat-sen Memorial Hospital Sun Yat-sen University of Medical Sciences
- From:Chinese Journal of Cancer
2001;20(3):279-281
- CountryChina
- Language:Chinese
-
Abstract:
Objectives：This study was designed to investigate the regulation effect of 8-Br-cAMP （ 8-Bromo 3′ ：5′ -cyclic adenosine monophosphate） on the expression of connexin gene (Cx43) in human tongue carcinoma cell line (Tca8113) . Methods：The inhibitory effect of 8-Br-cAMP on growth of Tca8113 cell line was examined by methyl thiazolyl tetrazolium (MTT) assay. Cx43 gene expression in Tca8113 cells after treated with 8-Br-cAMP was analyzed by immunocytochemistry and flow cytometry . Results：After treated with 8-Br-cAMP(10-5 mol/L, 10-4 mol/L, 10-3 mol/L), Tca8113 cells was inhibited and the inhibition rate was increased with the concentration of 8-Br-cAMP; Immunocytochemistry showed Cx43 protein detectability and up-regulation in 8-Br-cAMP treated cells; The positive rate of Cx43 protein in Tca8113 ce1ls increased from 4.8％ to 26.5％ . There was significant difference between 8-Br-cAMP treated cells and untreated cells （ P<0.01） . Conclusion： The anti-tumor effect of 8-Br-cAMP on Tca8113 cells might be due to up-regu1ation on Cx43 gene.
