BCRP Gene Expression in Normal Lung and in Non-Small Cell Lung Cancer Tissue
10.3321/j.issn:1000-467X.2001.03.011
- VernacularTitle:BCRP基因在非小细胞肺癌组织和非癌肺组织中的表达及意义
- Author:
You-Sheng MAO
1
;
Doyle AUSTIN
;
Yang WEIDONG
;
Wei YUETONG
;
J.Krasna MARK
;
D.Ross DOUGLAS
Author Information
1. Cancer Hospital Chinese Academy of Medical Sciences
- From:Chinese Journal of Cancer
2001;20(3):274-278
- CountryChina
- Language:Chinese
-
Abstract:
Objective：This study was designed to investigate BCRP gene expression in normal lung and in non-small cell lung cancer tissue. Methods：RNA was extracted immediately from the fresh normal lung tissue and viable tumor tissue harvested from surgically resected specimens of non-small cell lung cancer patients. cDNA of BCRP gene was prepared by RT-PCR and then amplified by PCR. The cDNA products from those specimens were transferred to blotting membrane through electrophoresis and transferring technique. Southern blot hybridization was eventually performed to detect the expression of BCRP gene. Results：RNAs were extracted from 8 tumor tissues and 12 pairs of tumor tissue and normal lung tissue were harvested from the same lung. Four patients’RNA samples with poor quality due to degrading were discarded. cDNA products of BCRP gene were obtained by RT-PCR and amplified by PCR in the remain 16 patients’RNA samples. Through Southern blot hybridization, BCRP gene was found to have slight expression in various amounts in all normal lung tissue(10/10) and only in half of tumor tissue samples (8/16). Conclusion：BCRP gene is slightly expressed in a variable amount in all normal lung tissue and only in half of tumor tissue for this group of patients with non-small cell lung cancer. Therefore chemotherapy with anthracycline may possibly induce the overexpression of BCRP gene and at last to develop multidrug resistance.
