Molecular etiological study on the GI.6 Norovirus causing an infectious diarrhea outbreak in Shenyang, China
10.3760/cma.j.cn112866-20230403-00033
- VernacularTitle:我国沈阳一起GI.6型札如病毒引起的胃肠炎暴发的分子病原学特征研究
- Author:
Jun TIAN
1
;
Shi CONG
;
Ling CHEN
;
Jiayuan LIANG
;
Bing WANG
Author Information
1. 大连市第三人民医院，大连 116000
- Keywords:
Norovirus;
Infectious diarrhea outbreak;
GI.6 genotype;
Genetic evolution
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(3):310-314
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the molecular pathogenic characteristics and genetic evolution of an outbreak of acute gastroenteritis in Shenyang, China.Methods:A total of 57 anal swabs and environmental swab samples from student cases in a university outbreak in Shenyang in November 2021 were collected. Fluorescence quantitative RT-PCR was used for routine detection of diarrheal virus genes, and traditional RT-PCR was used to amplify and sequence the positive specimens of Norovirus. Phylogenetic analysis was performed.Results:Of the 57 specimens, 26 were norovirus positive by fluorescence quantitative real-time PCR, and 15 specimens were successfully amplified for the capsid protein gene segment. Phylogenetic analysis showed that all 15 positive specimens were of the GI.6 genotype of Norovirus, with a homology of 99.99% among the specimens and a genetic similarity of 98.84% to the LC380411 strain in Japan in 2005.Conclusions:The pathogen of this outbreak, GI.6 Norovirus, is rarely detected in the northeast of China, and its genotype is a subtype that has rarely been reported worldwide.
