Apoptosis Induction in Tumor Cells by Photosensitizer Mesotetrahydroxyphenylchlorin
10.3321/j.issn:1000-467X.2001.03.008
- VernacularTitle:光敏剂间-四羟基苯二氢卟酚诱导肿瘤细胞凋亡的研究
- Author:
Jian-Ming WEN
1
;
Lin WANG
;
Meng ZHANG
;
Dan XIE
Author Information
1. Sun Yat-sen University of Medical Sciences
- From:Chinese Journal of Cancer
2001;20(3):262-265
- CountryChina
- Language:Chinese
-
Abstract:
Objective：This study was designed to investigate the death mechanism of tumor cells after photodynamic therapy with mesotetrahydroxyphenylchlorin(mTHPC) as a photosensitizer. Methods：Human osteosarcoma cells (HOS-8603) and murine myeloid leukemia cells(JCS) were treated with 1.3 μ mol/L mTHPC for 16 hours in vitro, and then exposed to 7.5 mW/cm2 intensity of 580 nm light. Cell survival was calculated and intracellular localization of mTHPC was observed by confocal laser scanninig microscopy. Apoptotic morphology of the cells was demonstrated by fluorescence staining of Hoechst 33258. Apoptotic DNA ladders were detected by agarose electrophoresis. Results：Fluorescence of mTHPC was observed in the cytoplasm of two cell types. After photodynamic therapy, cell survival rate was decreased with prolongation of incubation time. Fifty percent of cell killing was at 2 hours in JCS cells, and at 4 hours in HOS-8603 cells after light treatment. The percentages of apoptotic cells in JCS were 2.1％ at 1 h, 62.9％ at 2 h and 100％ at 4 h, and in HOS-8603 were 20.2％ at 4 h, 65.9％ at 8 h and 100％ at 12 h, respectively. A characteristic apoptotic ladders were showed in all cell types. Conclusion：The mTHPC is a potent photosensitizer and can penetrate into cytoplasm of the cells effectively. Induced cell death of photodynamic therapy is mainly apoptosis followed by necrosis.
