Transcriptomic analysis of human papillomavirus type 16 immortalized cervical epithelial cell line H8 after malignant transformation
10.3760/cma.j.cn112866-20211222-00213
- VernacularTitle:人乳头瘤病毒16整合感染宫颈上皮细胞系恶性转化后的转录组学分析
- Author:
Yi TANG
1
;
Quan CHEN
;
Huaping LI
;
Runxiang LI
;
Bihua LIANG
;
Liqian PENG
;
Jiaoquan CHEN
;
Shanshan OU
;
Weihong WU
;
Huilan ZHU
Author Information
1. 广州医科大学附属皮肤病研究所，广州 510095
- Keywords:
RNA-seq;
Transcriptomic;
Human Papillomavirus 16;
Malignant transformation;
Immortalized human cervical epithelial cells
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(3):303-309
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the related genes, signaling pathways and possible mechanisms of malignant transformation of human papillomavirus type 16 (HPV-16) immortalized cervical epithelial cell line H8.Methods:The malignant transformed H8 cell model was constructed, and the changes of cell invasion ability and cell migration ability of H8 cells after malignant transformation were detected by Transwell assay, and the changes of clone formation ability of H8 cells after malignant transformation were detected by plate clone formation assay. Total RNA was extracted from malignant transformed H8 cells and H8 cells, and the two groups of cells were sequenced by transcriptome using Illumina novaseq 6000 sequencing platform, differentially expressed genes (DEGs) were identified and analyzed, and Gene Ontology (GO) function enrichment analysis, Kyoto Encyclopedia of genes and genomes (KEGG) pathway enrichment analysis and protein-protein interaction were performed.Results:The invasion ability, migration ability and clone formation ability of malignant transformed H8 cells significantly increased as compared to H8 cells. A total of 203 differentially expressed genes were identified in H8 cells before and after malignant transformation, of which 98 were up-regulated and 105 down-regulated. GO enrichment analysis showed that DEGs were mainly involved in biological processes such as cellular processes, biological regulation, and metabolic processes. KEGG pathway enrichment analysis showed that DEGs were mainly enriched in alanine, aspartate and glutamate metabolic pathway, glycine, serine and threonine metabolism pathway, p53 signaling pathway and TGF-β signaling pathway, PI3K-Akt signaling pathway. PPI analysis screened 10 hub genes including DDIT3, TRIB3 and ASNS.Conclusions:Compared with H8 cells, malignant transformed H8 cells have a large number of differentially expressed genes and pathways at the transcriptional level, which could further provide new ideas for the mechanism of malignant transformation and carcinogenesis as well as finding new targets for the prevention of malignant transformation.
