Application of genome-wide genechip for screening and identifying genes related to CD133+CD200+colorectal cancer stem cells
10.3969/j.issn.1673-4254.2013.12.17
- VernacularTitle:基因芯片技术筛选和鉴定CD133+CD200+大肠癌干细胞相关基因
- Author:
Shanshan ZHANG
1
;
Lixuan LI
;
Zaiwei HUANG
;
Xiaomin XIN
;
Bing XIAO
Author Information
1. 南方医科大学南方医院消化科//广东省胃肠疾病重点实验室
- Keywords:
CD133;
CD200;
colorectal cancer stem cells;
genechip;
MAPK signaling pathway
- From:
Journal of Southern Medical University
2013;(12):1787-1791
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and identity genes related to CD133+CD200+ colorectal cancer stem cells. Methods The two subpopulations of colorectal cancer cells, namely CD133+CD200+ and CD133-CD200- cells, were sorted and verified by flow cytometry. The gene expression profiles of CD133 +CD200 +and CD133-CD200- colorectal cancer cells were examined using Affymetrix Human U133 Plus2.0 genome- wide genechip. The differentially expressed genes between the two cell subpopulations were analyzed to identify the genes responsible for the main effect in association with colorectal cancer stem cells. Real-time quantitative PCR was performed to confirm some of the differentially expressed genes identified by genechip. Results The genechip result showed that 655 genes were differentially expressed in CD133+CD200+colorectal cancer stem cells by at least 3 folds, including 290 up-regulated and 365 down-regulated ones. Bioinformatics analysis and gene co-expression network building identified 3 genes (MDM2, PRKACG, and CACNA1G) with specific expression in CD133+CD200+colorectal cancer stem cells, and this result was confirmed by real-time quantitative PCR analysis. Conclusion A specific gene expression profile of colorectal cancer stem cells has been established through screening and identifying genes related to CD133+CD200+colorectal cancer stem cells by gene genechip technique, which provides a basis for further study of gene targeting therapy of colorectal cancer.
